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Mutation screening by MLPA. MLPA analysis was used to detect large deletions or insertions in the FANCA gene. An MLPA kit for the FANCA gene, SALSA P031/P032, was used in this study (MRC-Holland, Amesterdam, Netherlands). Briefly, DNA was denatured for 5 min at 98°C, 3 μL of the probe mix was added, after which the mixture was heated for 1 min at 98°C and incubated at 60°C overnight (16 h).
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